Loss of Mir146b with aging contributes to inflammation and mitochondrial dysfunction in thioglycollate-elicited peritoneal macrophages

Macrophages undergo programmatic changes with age, leading to altered cytokine polarization and immune dysfunction, shifting these critical cells from protective sentinels disease promoters. The molecular mechanisms underlying macrophage inflammaging are poorly understood. Using an unbiased RNA sequencing (RNA-seq) approach, we identified Mir146b as a microRNA whose expression progressively unidirectionally declined age in thioglycollate-elicited murine macrophages. deficiency led reduced mitochondrial metabolic activity, two hallmarks of cellular aging. Single-cell RNA-seq patterns inflammation interferon gamma signaling Mir146b- deficient Identification potential regulator aging provides novel insights into dysfunction associated